Hi K.! I'm an Ultrasound tech and have assisted in many amnio procedures. Usually if the doctor wants to do it he's/she's just trying to gather more information about the health of your baby (i.e. if the lungs are developed enough for an early delivery...) It's not all about Down Syndrome. Of all the amnios I've witnessed, I've never seen a women in pain. They check the baby for his/her position, find a nice big pocket of fluid, they keep the ultrasound on your belly at all times, then they take about an ounce or 2 of fluid (it only takes a second) and then they take another look at the baby to check the heart rate (And give you another little peek) It's very safe and shouldn't hurt at all and if everything else in your genetic screening has turned out just fine I'm sure you have nothing to worry about, the Doctor probably just wants to be thorough, and it's a very fast and easy procedure. It's completely up to you but I'd say go for it! :-)